Abstract
BACKGROUND: Sesame is an important crop due to the excellent quality oil derived from its grains. With as any other crop, the seed used as initial input in the agricultural process, is the first warranty of success that a farmer has. The quality seed must be evaluated during the multiplication process, to be sure that varietal purity, as component of genetic quality, is not affected. OBJECTIVES: The general objective was to determine ability of random amplified polymorphic DNA (RAPD) markers to evaluate varietal purity; two specific objectives were defined: to evaluate the ability of RAPD to detect seeds of non-target cultivars (seed contaminants), and to evaluate the ability of RAPD to detect troubles during seed multiplication. MATERIAL AND METHODS: For first objective seeds of cultivar UCLA-1 were mixed with seeds of another known accession, in different proportions: 99:1, 98:2, 97:3 and 95:5. RAPD markers were performed on these different proportions, and band pattern of each proportion was compared to UCLA-1 and to the other accessions band pattern. For second objective, RAPD was performed on samples from genetic seed, and seeds of the two following multiplication cycles. RESULTS: RAPD was able to identify seed contaminant in proportion as low as 1%. For second objective, RAPD identified the three samples from genetic seed with the same band pattern, but it was different to band pattern of the other two kinds of seed. Therefore, RAPD had the ability to identify that the multiplication of seeds was not totally suitable, because some non-target seeds appeared as source of contamination. CONCLUSION: RAPD proved to be an effective tool to monitor genetic quality, specifically varietal purity, in sesame seed.
Keywords:
DNA, genetic quality, molecular marker, polymorphism, random amplified polymorphic DNA, seed qualityReferences
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